pcDNA GAL4 CREB 1-341
(Plasmid
#22972)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22972 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA
- Backbone size w/o insert (bp) 5000
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCREB
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SpeciesR. norvegicus (rat); GAL4-fusion
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Tag
/ Fusion Protein
- GAL4 DNA-binding domain (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
GAL4 DNA-binding domain fused N-terminally to full-length CREB cDNA (aa 1-341)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA GAL4 CREB 1-341 was a gift from Marc Montminy (Addgene plasmid # 22972 ; http://n2t.net/addgene:22972 ; RRID:Addgene_22972) -
For your References section:
Genome-wide analysis of CREB target genes reveals a core promoter requirement for cAMP responsiveness. Conkright MD, Guzman E, Flechner L, Su AI, Hogenesch JB, Montminy M. Mol Cell. 2003 Apr . 11(4):1101-8. 10.1016/S1097-2765(03)00134-5 PubMed 12718894