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pLL5.0_shRNAHsCavin1_Del4UC1_EGFP
(Plasmid #229691)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229691 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLL5.0
  • Backbone size w/o insert (bp) 7568
  • Vector type
    Lentiviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    shRNA for hCavin1
  • Alt name
    CGL4; PTRF; CAVIN; FKSG13; cavin-1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    75
  • Entrez Gene
    CAVIN1 (a.k.a. CAVIN, CGL4, FKSG13, PTRF, cavin-1)
  • Promoter LTR viral promoter
  • Tag / Fusion Protein
    • EGFP (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning

Gene/Insert 2

  • Gene/Insert name
    mutant cavin1b (Del4UC1)
  • Alt name
    ptrf; ptrfb; fd20g07; wu:fd20g07; zgc:162917
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1209
  • Mutation
    Del4UC1
  • Entrez Gene
    cavin1b (a.k.a. fd20g07, ptrf, ptrfb, wu:fd20g07, zgc:162917)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

hCAVIN1 shRNA: TCACCTTCCACGTCAAGAAGATCCGCGAGGTTCAAGAGACCTCGCGGATCTTCTTGACGTGGAAGGTGTTTTTTC

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLL5.0_shRNAHsCavin1_Del4UC1_EGFP was a gift from Alpha Yap (Addgene plasmid # 229691 ; http://n2t.net/addgene:229691 ; RRID:Addgene_229691)

  • For your References section:

    Caveola mechanotransduction reinforces the cortical cytoskeleton to promote epithelial resilience. Brooks JW, Tillu V, Eckert J, Verma S, Collins BM, Parton RG, Yap AS. Mol Biol Cell. 2023 Nov 1;34(12):ar120. doi: 10.1091/mbc.E23-05-0163. Epub 2023 Sep 6. 10.1091/mbc.E23-05-0163 PubMed 37672337
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