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Addgene

pCF CREB M1
(Plasmid #22969)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22969 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCF
  • Backbone size w/o insert (bp) 5000
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CREB
  • Species
    R. norvegicus (rat)
  • Mutation
    Changed Serine 133 to Alanine
  • Entrez Gene
    Creb1 (a.k.a. Creb)
  • Tag / Fusion Protein
    • FLAG (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCF CREB M1 was a gift from Marc Montminy (Addgene plasmid # 22969 ; http://n2t.net/addgene:22969 ; RRID:Addgene_22969)

  • For your References section:

    Characterization of a CREB gain-of-function mutant with constitutive transcriptional activity in vivo. Du K, Asahara H, Jhala US, Wagner BL, Montminy M. Mol Cell Biol. 2000 Jun . 20(12):4320-7. 10.1128/MCB.20.12.4320-4327.2000 PubMed 10825195
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