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Addgene

pCMV-eA3A-BE5-(NG)-PX458-EGFP
(Plasmid #229687)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229687 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    p2T-CMV-eA3A-BE5-NG-BlastR
  • Backbone manufacturer
    David Liu, Addgene plasmid #152998
  • Vector type
    Mammalian Expression, CRISPR
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • EGFP (C terminal on backbone)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    sgRNA cloning site taken from PX458 (Feng Zhang, Addgene plasmid #48138)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Alternative shortened plasmid name: eA3A-sgRNA-EGFP; plasmid size 10,326 bp

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCMV-eA3A-BE5-(NG)-PX458-EGFP was a gift from Deborah Marsh (Addgene plasmid # 229687 ; http://n2t.net/addgene:229687 ; RRID:Addgene_229687)

  • For your References section:

    CRISPR single base-editing: in silico predictions to variant clonal cell lines. Dickson KA, Field N, Blackman T, Ma Y, Xie T, Kurangil E, Idrees S, Rathnayake SNH, Mahbub RM, Faiz A, Marsh DJ. Hum Mol Genet. 2023 Jun 27:ddad105. doi: 10.1093/hmg/ddad105. 10.1093/hmg/ddad105 PubMed 37369005
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