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Addgene

Myc-G4-ND2 (N67)
(Plasmid #22967)

Full plasmid sequence is not available for this item.

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22967 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pRK5
  • Backbone size w/o insert (bp) 4754
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    DH10B
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    NeuroD2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1143
  • Entrez Gene
    Neurod2 (a.k.a. Ndrf, bHLHa1)
  • Tags / Fusion Proteins
    • Myc (N terminal on insert)
    • Gal4 DBD (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Myc-G4-ND2 (N67) was a gift from Anirvan Ghosh (Addgene plasmid # 22967 ; http://n2t.net/addgene:22967 ; RRID:Addgene_22967)