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Addgene

pEGFP-C1-mApg5(K130R)
(Plasmid #22959)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22959 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    autophagy related genes 5
  • Alt name
    Atg5
  • Alt name
    Apg5
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    830
  • Mutation
    130 Lysine to Arginine
  • GenBank ID
    AB048349
  • Entrez Gene
    Atg5 (a.k.a. 2010107M05Rik, 3110067M24Rik, Apg5l, Atg5l, Paddy)
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (destroyed during cloning)
  • 3′ cloning site SalI (destroyed during cloning)
  • 5′ sequencing primer EGFP-C
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEGFP-C1-mApg5(K130R) was a gift from Noboru Mizushima (Addgene plasmid # 22959 ; http://n2t.net/addgene:22959 ; RRID:Addgene_22959)

  • For your References section:

    Dissection of autophagosome formation using Apg5-deficient mouse embryonic stem cells. Mizushima N, Yamamoto A, Hatano M, Kobayashi Y, Kabeya Y, Suzuki K, Tokuhisa T, Ohsumi Y, Yoshimori T. J Cell Biol. 2001 Feb 19. 152(4):657-68. 10.1083/jcb.152.4.657 PubMed 11266458
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