pcDNA-Malibu-mScarlet
(Plasmid
#229554)
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PurposeFluorescent reporter for malonyl-CoA
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 229554 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepcDNA3.1
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameMalibu-mScarlet
- Promoter CMV
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Tag
/ Fusion Protein
- mScarlet (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV forward (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit ttps://doi.org/10.1101/2024.09.27.615526 for bioRxiv preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pcDNA-Malibu-mScarlet was a gift from Danielle Schmitt (Addgene plasmid # 229554 ; http://n2t.net/addgene:229554 ; RRID:Addgene_229554) -
For your References section:
A Genetically Encoded Fluorescent Biosensor for Intracellular Measurement of Malonyl-CoA. Ranzau BL, Robinson TD, Scully JM, Kapelczack ED, Dean TS, TeSlaa T, Schmitt DL. bioRxiv [Preprint]. 2024 Sep 28:2024.09.27.615526. doi: 10.1101/2024.09.27.615526. 10.1101/2024.09.27.615526 PubMed 39386450
Map uploaded by the depositor.
