pMVRha
(Plasmid
#229553)
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Purpose(Empty Backbone) Broad-host range plasmid (pBBR1), contains an oriT (RP4) for conjugation in kanamycin sensitive non-model bacteria. The GOI can be cloned under the control of a rhamnose-inducible promoter using BsaI.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 229553 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepCAT201
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Backbone manufacturerThomas Howard (Addgene plasmid #134878)
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Modifications to backboneThe backbone was only used as source of the replicon and marker region.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsConstitutive spisPink expression causes metabolic burden. E. coli strains containing this plasmid grow slower than the wild type strain.
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Copy numberLow Copy
Cloning Information
- Cloning method Golden Gate
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMVRha was a gift from Sandy Schmidt (Addgene plasmid # 229553 ; http://n2t.net/addgene:229553 ; RRID:Addgene_229553) -
For your References section:
Using Cupriavidus necator H16 to Provide a Roadmap for Increasing Electroporation Efficiency in Nonmodel Bacteria. Vajente M, Clerici R, Ballerstedt H, Blank LM, Schmidt S. ACS Synth Biol. 2024 Oct 31. doi: 10.1021/acssynbio.4c00380. 10.1021/acssynbio.4c00380 PubMed 39482869
Map uploaded by the depositor.
