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lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE
(Plasmid #229544)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229544 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    custom backbone
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Kanamycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Oct4(co)-mNeonGreen
  • Species
    H. sapiens (human)
  • Mutation
    codon-optimized OCT4
  • Entrez Gene
    POU5F1 (a.k.a. OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4)
  • Promoter TRE12
  • Tag / Fusion Protein
    • Oct4-mNeonGreen

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lenti_U6_miR-Oct4e_TRE12_OCT4(co)-mNeonGreen_WPRE was a gift from Domitilla Del Vecchio (Addgene plasmid # 229544 ; http://n2t.net/addgene:229544 ; RRID:Addgene_229544)

  • For your References section:

    Synthetic genetic circuits to uncover the OCT4 trajectories of successful reprogramming of human fibroblasts. Ilia K, Shakiba N, Bingham T, Jones RD, Kaminski MM, Aravera E, Bruno S, Palacios S, Weiss R, Collins JJ, Del Vecchio D, Schlaeger TM. Sci Adv. 2023 Dec;9(48):eadg8495. doi: 10.1126/sciadv.adg8495. Epub 2023 Nov 29. 10.1126/sciadv.adg8495 PubMed 38019912
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