pOPINB-M1-di-Ub(G76V)-HOIL-1
(Plasmid #229539)

• Purpose: Constitutively active M1-di-ubiquitin HOIL-1 fusion protein for expression in E. coli
• Depositing Lab: Bernhard Lechtenberg
• Publication: Wang et al bioRxiv November 30, 2024

Backbone

• Vector backbone: pOPINB
• Backbone size w/o insert (bp): 5642
• Total vector size (bp): 7299
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: Ubiquitin
• Alt name: Polyubiquitin
• Species: H. sapiens (human)
• Mutation: Changed Gly 76 to Val
• Entrez Gene: UBC (a.k.a. HMG20)
• Promoter: T7
• Tag / Fusion Protein:
  • 6xHis-3C (N terminal on backbone)

Cloning Information for Gene/Insert 1

• Cloning method: Other
• 5′ sequencing primer: T7 forward

Gene/Insert 2

• Gene/Insert name: Ubiquitin
• Alt name: Polyubiquitin
• Species: H. sapiens (human)
• Mutation: changed Gly 76 to Val
• Entrez Gene: UBC (a.k.a. HMG20)

Gene/Insert 3

• Gene/Insert name: HOIL-1
• Alt name: RBCK1
• Species: H. sapiens (human)
• Entrez Gene: RBCK1 (a.k.a. C20orf18, HOIL-1, HOIL1, PBMEI, PGBM1, RBCK2, RNF54, UBCE7IP3, XAP3, XAP4, ZRANB4)

Resource Information

• Supplemental Documents:
  • BCL1784_M1-di-UBG76V_HOIL-1_pOPINB.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This insert encodes an N-terminal 6xHis-tag with a 3C protease cleavage site, 2 ubiquitin molecules each with a G76V mutation fused to human HOIL-1.
All sequences codon-optimised for bacterial expression.