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Addgene

pCas3
(Plasmid #229538)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229538 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMV_hyg
  • Backbone size w/o insert (bp) 4778
  • Total vector size (bp) 9244
  • Vector type
    Bacterial Expression, Yeast Expression, CRISPR
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Cas3
  • Species
    E. coli
  • Insert Size (bp)
    2664
  • Mutation
    codon optimized for S.cerevisiae

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCas3 was a gift from Kevin Verstrepen (Addgene plasmid # 229538 ; http://n2t.net/addgene:229538 ; RRID:Addgene_229538)

  • For your References section:

    A Cas3-base editing tool for targetable in vivo mutagenesis. Zimmermann A, Prieto-Vivas JE, Cautereels C, Gorkovskiy A, Steensels J, Van de Peer Y, Verstrepen KJ. Nat Commun. 2023 Jun 9;14(1):3389. doi: 10.1038/s41467-023-39087-z. 10.1038/s41467-023-39087-z PubMed 37296137
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