pCas3-CDA1
(Plasmid #229534)

• Purpose: pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning
• Depositing Lab: Kevin Verstrepen
• Publication: Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi: 10.1038/s41467-023-39087-z.

Backbone

• Vector backbone: pMV_hyg
• Vector type: Bacterial Expression, Yeast Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: Cas3
• Species: E.coli
• Insert Size (bp): 2664
• Mutation: codon optimized for S.cerevisiae.
• Tag / Fusion Protein:
  • XTEN (C terminal on insert)

Gene/Insert 2

• Gene/Insert name: Cytidine deaminase
• Alt name: pmCDA1
• Species: Petromyzon marinus
• Insert Size (bp): 624
• Mutation: codon optimized for S.cerevisiae

Gene/Insert 3

• Gene/Insert name: Uracil glycosylase inhibitor
• Alt name: UGI
• Species: bateriophage PBS2
• Insert Size (bp): 252

Resource Information

• Supplemental Documents:
  • pCas3-CDA1.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry