pBHM2643
(Plasmid
#229527)
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PurposeCarries OsTIR1-t2a-mNG-mAID_opt, used to insert TIR1 expressing construct into the C. neoformans genome.
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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229527 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRS316
- Backbone size w/o insert (bp) 4839
- Total vector size (bp) 11508
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Vector typeBacterial Expression, Yeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOsTIR1
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Alt nameCryptococcus neoformans codon optimized TIR1 from Oryza sativa
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Insert Size (bp)6657
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MutationF74G
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Tag
/ Fusion Protein
- t2a-mNeonGreen-mAID_opt (C terminal on insert)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pBHM2643 was a gift from Hiten Madhani (Addgene plasmid # 229527 ; http://n2t.net/addgene:229527 ; RRID:Addgene_229527)