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Addgene

pCI-neo-hApg5(K130R)-HA
(Plasmid #22949)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22949 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCI-neo
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Homo sapiens ATG5 autophagy related 5 homolog (S. cerevisiae)
  • Alt name
    Atg5
  • Alt name
    Apg5
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    830
  • Mutation
    130 Lysine to Arginine
  • Entrez Gene
    ATG5 (a.k.a. APG5, APG5-LIKE, APG5L, ASP, SCAR25, hAPG5)
  • Tag / Fusion Protein
    • 3xHA (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SmaI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer T3
  • 3′ sequencing primer T7
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI-neo-hApg5(K130R)-HA was a gift from Noboru Mizushima (Addgene plasmid # 22949 ; http://n2t.net/addgene:22949 ; RRID:Addgene_22949)

  • For your References section:

    A new protein conjugation system in human. The counterpart of the yeast Apg12p conjugation system essential for autophagy. Mizushima N, Sugita H, Yoshimori T, Ohsumi Y. J Biol Chem. 1998 Dec 18. 273(51):33889-92. 10.1074/jbc.273.51.33889 PubMed 9852036
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