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Addgene

JD150
(Plasmid #229485)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229485 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pTwist high copy
  • Backbone manufacturer
    Twist Bioscience
  • Backbone size w/o insert (bp) 5835
  • Vector type
    Mammalian Expression, Adenoviral, AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    E2A, VA RNA I, E4orf6, L4-22k/33k
  • Species
    Synthetic
  • Insert Size (bp)
    4797
  • Promoter CMV (E2A, E4orf6), Ef1a (L4-22k) and wt promoter (VA RNA I)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' Cloning Site: HpaI (Destroyed), 3' Cloning Site: HpaI (Destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    JD150 was a gift from Nerea Zabaleta (Addgene plasmid # 229485 ; http://n2t.net/addgene:229485 ; RRID:Addgene_229485)

  • For your References section:

    E2A, VA RNA I, and L4-22k adenoviral helper genes are sufficient for AAV production in HEK293 cells. Doshi J., Couto E., Staiti J., Vandenberghe L.H., Zabaleta N.. Molecular Therapy Methods & Clinical Development, Volume 32, Issue 4, 101376 10.1016/j.omtm.2024.101376
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