Skip to main content
Addgene

pBD1
(Plasmid #229463)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229463 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pOKD5
  • Backbone size w/o insert (bp) 3787
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    RssB
  • Species
    E. coli
  • Insert Size (bp)
    1012
  • Promoter T7

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Susan Gottesman Lab: Insert was amplified by PCR from plasmid pT7-RssB, whose construction was reported in Zhou et al. J Bacteriol. 2006 Oct;188(19):7022-5. doi: 10.1128/JB.00687-06 and Zhou et al. Genes Dev. 2001 Mar 1;15(5):627-37. doi: 10.1101/gad.864401.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBD1 was a gift from Alexandra Deaconescu (Addgene plasmid # 229463 ; http://n2t.net/addgene:229463 ; RRID:Addgene_229463)

  • For your References section:

    Structural basis for inhibition of a response regulator of sigma(S) stability by a ClpXP antiadaptor. Dorich V, Brugger C, Tripathi A, Hoskins JR, Tong S, Suhanovsky MM, Sastry A, Wickner S, Gottesman S, Deaconescu AM. Genes Dev. 2019 Jun 1;33(11-12):718-732. doi: 10.1101/gad.320168.118. Epub 2019 Apr 11. 10.1101/gad.320168.118 PubMed 30975721
Related items: