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Addgene

pMS18
(Plasmid #229462)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229462 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSKB2
  • Backbone size w/o insert (bp) 5368
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab recommends OneShot Top10 for plasmid storage and propagation and BL21 DE3 for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IraD
  • Species
    E. coli
  • Insert Size (bp)
    456
  • Mutation
    Q68A
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Other

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMS18 was a gift from Alexandra Deaconescu (Addgene plasmid # 229462 ; http://n2t.net/addgene:229462 ; RRID:Addgene_229462)

  • For your References section:

    Structural basis for inhibition of a response regulator of sigma(S) stability by a ClpXP antiadaptor. Dorich V, Brugger C, Tripathi A, Hoskins JR, Tong S, Suhanovsky MM, Sastry A, Wickner S, Gottesman S, Deaconescu AM. Genes Dev. 2019 Jun 1;33(11-12):718-732. doi: 10.1101/gad.320168.118. Epub 2019 Apr 11. 10.1101/gad.320168.118 PubMed 30975721
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