Skip to main content
Addgene

pVF14
(Plasmid #229455)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229455 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pSKB2
  • Backbone size w/o insert (bp) 5368
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    The depositing lab recommends DH5alpha T1R for plasmid storage and propagation and BL21 DE3 for protein expression.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    IraD
  • Species
    E. coli
  • Insert Size (bp)
    456
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Susan Gottesman Lab: Insert was amplified by PCR from plasmid pQE80L‐IraD‐His6, whose construction was reported in Bougdour et al. Mol Microbiol. 2008 Apr;68(2):298-313. doi: 10.1111/j.1365-2958.2008.06146.x.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pVF14 was a gift from Alexandra Deaconescu (Addgene plasmid # 229455 ; http://n2t.net/addgene:229455 ; RRID:Addgene_229455)

  • For your References section:

    Structural basis for inhibition of a response regulator of sigma(S) stability by a ClpXP antiadaptor. Dorich V, Brugger C, Tripathi A, Hoskins JR, Tong S, Suhanovsky MM, Sastry A, Wickner S, Gottesman S, Deaconescu AM. Genes Dev. 2019 Jun 1;33(11-12):718-732. doi: 10.1101/gad.320168.118. Epub 2019 Apr 11. 10.1101/gad.320168.118 PubMed 30975721
Related items: