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Purpose3rd generation lentiviral vector encoding human p53 with a C-terminal V5 tag
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22945 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepLenti6/V5-D-TOPO
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 6963
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Vector typeMammalian Expression, Lentiviral
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Copy numberUnknown
Gene/Insert
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Gene/Insert nametumor protein 53
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Alt nameTP53
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SpeciesH. sapiens (human)
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Insert Size (bp)1179
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Entrez GeneTP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
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Tag
/ Fusion Protein
- V5 (C terminal on backbone)
Cloning Information
- Cloning method TOPO Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer pBABE-3 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti6/V5-p53_wt p53 was a gift from Bernard Futscher (Addgene plasmid # 22945 ; http://n2t.net/addgene:22945 ; RRID:Addgene_22945) -
For your References section:
Different mutant/wild-type p53 combinations cause a spectrum of increased invasive potential in nonmalignant immortalized human mammary epithelial cells. Junk DJ, Vrba L, Watts GS, Oshiro MM, Martinez JD, Futscher BW. Neoplasia. 2008 May . 10(5):450-61. PubMed 18472962
