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pLenti6/V5-p53_R175H
(Plasmid #22936)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22936 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLenti6/V5-D-Topo
  • Backbone size w/o insert (bp) 6963
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Growth instructions
    Try using 50 ug/ml ampicillin if the bacteria do not grow well at 100 ug/ml.
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    tumor protein 53
  • Alt name
    TP53
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1179
  • Mutation
    R175H Mutant
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Tag / Fusion Protein
    • V5 (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti6/V5-p53_R175H was a gift from Bernard Futscher (Addgene plasmid # 22936 ; http://n2t.net/addgene:22936 ; RRID:Addgene_22936)

  • For your References section:

    Different mutant/wild-type p53 combinations cause a spectrum of increased invasive potential in nonmalignant immortalized human mammary epithelial cells. Junk DJ, Vrba L, Watts GS, Oshiro MM, Martinez JD, Futscher BW. Neoplasia. 2008 May . 10(5):450-61. PubMed 18472962
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