pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC
(Plasmid #22931)

• Purpose: Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)
• Depositing Lab: Alexandra Newton
• Publication: Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Modifications to backbone: Created by removing last 3aa from pcDNA3-HA-PHLPP1 N-terminal truncation (Addgene Plasmid #22404)
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PHLPP1-deltaC
• Alt name: SCOP
• Species: H. sapiens (human)
• Insert Size (bp): 3606
• Mutation: PHLPP-deltaC, deletion of PDZ binding domain--the last three amino acid residues 1203-1205.
• Entrez Gene: PHLPP1 (a.k.a. PHLPP, PLEKHE1, PPM3A, SCOP)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid was created using PHLPP1 N-terminal truncation as template. Please note that PHLPP1 N-terminal truncation was referred to as PHLPP1alpha in the original publication (Gao et al. Mol Cell 2015)