pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH
(Plasmid #22930)

• Purpose: Mammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domain
• Depositing Lab: Alexandra Newton
• Publication: Gao et al Mol Cell. 2005 Apr 1. 18(1):13-24.

Backbone

• Vector backbone: pcDNA3
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5446
• Modifications to backbone: Created by removing PH domain from pcDNA3-HA-PHLPP1 N-terminal truncation (Addgene Plasmid #22404)
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PHLPP1-deltaPH
• Alt name: SCOP
• Species: H. sapiens (human)
• Insert Size (bp): 3234
• Mutation: Includes 127-1205 aa. Deleted amino-terminal PH domain
• Entrez Gene: PHLPP1 (a.k.a. PHLPP, PLEKHE1, PPM3A, SCOP)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: BGH-rev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasmid was created using PHLPP1 N-terminal truncation as template. Please note that PHLPP1 N-terminal truncation was referred to as PHLPP1 in the original publication (Gao et al., 2005) and PHLPP1alpha in the subsequent publication (Brognard et al., 2007; PMID:
17386267).