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Addgene

pCX459
(Plasmid #229214)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229214 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRISPR array plasmid for MAP1B (Spacers 1-24)
  • gRNA/shRNA sequence
    MAP1B 3'UTR
  • Species
    Synthetic

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.07.14.603457 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCX459 was a gift from Jennifer Doudna (Addgene plasmid # 229214 ; http://n2t.net/addgene:229214 ; RRID:Addgene_229214)

  • For your References section:

    Single-molecule live-cell RNA imaging with CRISPR–Csm. Xia C, Colognori D, Jiang X, Xu K, Doudna JA. Nat Biotechnol (2025) 10.1038/s41587-024-02540-5 PubMed 39071319
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