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Addgene

pTlpA_mCherry_YafQ/DinJ
(Plasmid #229148)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229148 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pLp_3050sNuc
  • Backbone manufacturer
    Addgene
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Erythromycin, 200 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mCherry, DinJ/YafQ
  • Species
    Discosoma spp, Lacticaseibacillus casei
  • Promoter PtlpA (mCherry), DinJ/YafQ (Native)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene observed a ~1.4k base pair insertion downstream of the T7 terminator. This discrepancy does not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTlpA_mCherry_YafQ/DinJ was a gift from Shrikrishnan Sankaran (Addgene plasmid # 229148 ; http://n2t.net/addgene:229148 ; RRID:Addgene_229148)

  • For your References section:

    Novel genetic modules encoding high-level antibiotic-free protein expression in probiotic lactobacilli. Dey S, Blanch-Asensio M, Balaji Kuttae S, Sankaran S. Microb Biotechnol. 2023 Jun;16(6):1264-1276. doi: 10.1111/1751-7915.14228. Epub 2023 Feb 1. 10.1111/1751-7915.14228 PubMed 36722614
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