HR700_TP53 Exon Mutation Libraries
(Pooled Libraries #229137, #229138, #229139, #229140)
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Purpose
Donor vector libraries for CRISPR/Cas9-mediated saturation genome editing of TP53 exons 5–8. Each library includes all single nucleotide substitutions and additional missense, nonsense, and synonymous variants requiring 2 or 3 nucleotide changes, single-nucleotide insertions, 1–3 bp deletions for each TP53 exon 5 through 8, and 12 flanking intronic nucleotides.
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Vector Backbone
- #229137: HR700_HA2TP53_Ex5 (Plasmid #228860)
- #229138: HR700_HA2TP53_Ex6 (Plasmid #228861)
- #229139: HR700_HA2TP53_Ex7 (Plasmid #228862)
- #229140: HR700_HA2TP53_Ex8 (Plasmid #228863)
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Depositing Labs
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |||
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Pooled Library | 229137 | HR700_TP53Exon5mut | 1 | $380 | Add to Cart | ||
Pooled Library | 229138 | HR700_TP53Exon6mut | 1 | $380 | Add to Cart | ||
Pooled Library | 229139 | HR700_TP53Exon7mut | 1 | $380 | Add to Cart | ||
Pooled Library | 229140 | HR700_TP53Exon8mut | 1 | $380 | Add to Cart |
Library Details
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SpeciesHuman
Pooled Library | Variants Per Library | Size of insert |
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229137 | 3,034 | 208 nucleotides |
229138 | 1,963 | 137 nucleotides |
229139 | 1,910 | 134 nucleotides |
229140 | 2,323 | 161 nucleotides |
Library Shipping
Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.
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Volume∼15 µL
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
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Scripts
- The code (Link opens in a new window) related to these libraries is available on GitHub.
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Terms and Licenses
Academic/Nonprofit TermsIndustry TermsTrademarks- Zeocin® is an InvivoGen trademark.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
HR700_TP53Exon5mut library was a gift from Thorsten Stiewe (Addgene #229137)
HR700_TP53Exon6mut library was a gift from Thorsten Stiewe (Addgene #229138)
HR700_TP53Exon7mut library was a gift from Thorsten Stiewe (Addgene #229139)
HR700_TP53Exon8mut library was a gift from Thorsten Stiewe (Addgene #229140) -
For your References section:
Deep CRISPR mutagenesis characterizes the functional diversity of TP53 mutations. Funk JS, Klimovich M, Drangenstein D, Pielhoop O, Hunold P, Borowek A, Noeparast M, Pavlakis E, Neumann M, Balourdas DI, Kochhan K, Merle N, Bullwinkel I, Wanzel M, Elmshauser S, Teply-Szymanski J, Nist A, Procida T, Bartkuhn M, Humpert K, Mernberger M, Savai R, Soussi T, Joerger AC, Stiewe T. Nat Genet. 2025 Jan;57(1):140-153. doi: 10.1038/s41588-024-02039-4. Epub 2025 Jan 7. PubMed 39774325