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CAG-VEGF120-T2A-mCherry
(Plasmid #229134)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229134 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    CAG-GFP
  • Backbone manufacturer
    Addgene
  • Backbone size w/o insert (bp) 6800
  • Total vector size (bp) 7949
  • Modifications to backbone
    Inserted VEGF120 and replaced GFP with mCherry
  • Vector type
    Mammalian Expression, Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    VEGF120
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    438
  • Entrez Gene
    Vegfa (a.k.a. L-VEGF, Vegf, Vpf)

Gene/Insert 2

  • Gene/Insert name
    mCherry
  • Insert Size (bp)
    708

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.04.24.537801 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CAG-VEGF120-T2A-mCherry was a gift from Elizabeth Kirby (Addgene plasmid # 229134 ; http://n2t.net/addgene:229134 ; RRID:Addgene_229134)

  • For your References section:

    Neural stem and progenitor cells support and protect adult hippocampal function via vascular endothelial growth factor secretion. Miller LN, Walters AE, Denninger JK, Hanson MA, Marshall AH, Johantges AC, Hosawi M, Sebring G, Rieskamp JD, Ding T, Rindani R, Chen KS, Goldberg ME, Senthilvelan S, Volk A, Zhao F, Askwith C, Wester JC, Kirby ED. Mol Psychiatry. 2024 Nov 11. doi: 10.1038/s41380-024-02827-8. 10.1038/s41380-024-02827-8 PubMed 39528687
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