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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22913 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namefugu somatostatin promoter driving DsRed 2
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Speciestakifugu rubripes (fugu)
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Insert Size (bp)4300
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Depositor used a previous fugu genome sequence, and this may be different to the
current version.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-fSST-RFP was a gift from Edward Callaway (Addgene plasmid # 22913 ; http://n2t.net/addgene:22913 ; RRID:Addgene_22913) -
For your References section:
Short Promoters in Viral Vectors Drive Selective Expression in Mammalian Inhibitory Neurons, but do not Restrict Activity to Specific Inhibitory Cell-Types. Nathanson JL, Jappelli R, Scheeff ED, Manning G, Obata K, Brenner S, Callaway EM. Front Neural Circuits. 2009 . 3():19. 10.3389/neuro.04.019.2009 PubMed 19949461