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Depositing Lab
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Publication
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22908 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
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SerotypeSelect serotype for details See details about
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PricingSelect serotype and quantity $ USD for preparation of µL virus + $30 USD for plasmid.
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Backbone
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Vector backbonepAAV
- Backbone size w/o insert (bp) 4000
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Vector typeMammalian Expression, AAV
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)Stbl3
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Growth instructionsStbl3
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemouse alpha-calcium/calmodulin-dependent protein kinase II promoter
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SpeciesM. musculus (mouse)
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Insert Size (bp)3000
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Entrez GeneCamk2a (a.k.a. CaMKII, mKIAA0968)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer Unknown
- 3′ sequencing primer SP6 (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note- this plasmid contains the mouse α-calcium/calmodulin-dependent protein kinase II promoter (mCAMK). There are several mismatches between depositor's sequence and Addgene's sequence. Please refer to Addgene's results for the correct sequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAAV-mCAMK-RFP was a gift from Edward Callaway (Addgene plasmid # 22908 ; http://n2t.net/addgene:22908 ; RRID:Addgene_22908) -
For your References section:
Preferential labeling of inhibitory and excitatory cortical neurons by endogenous tropism of adeno-associated virus and lentivirus vectors. Nathanson JL, Yanagawa Y, Obata K, Callaway EM. Neuroscience. 2009 Jun 30. 161(2):441-50. 10.1016/j.neuroscience.2009.03.032 PubMed 19318117