pLentiCRISPR_mCherry
(Plasmid
#229078)
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Purpose(Empty Backbone) Small lentiviral backbone for CRISPR guide libraries, improved titre for hard to transduce cells
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 229078 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonelentiCRISPR v2
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Backbone manufacturerFeng Zhang
- Backbone size (bp) 7466
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Vector typeMammalian Expression, CRISPR
- Promoter EF1s
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Selectable markersZeocin ; mCherry
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This plasmid was made by and is submitted on behalf of Nutsa Burduli and Evren Alici.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLentiCRISPR_mCherry was a gift from Bernhard Schmierer (Addgene plasmid # 229078 ; http://n2t.net/addgene:229078 ; RRID:Addgene_229078)
