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Addgene

pmU1-CasRx-non-targeting (pKW83)
(Plasmid #229051)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 229051 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Use BbsI to insert the spacer sequence; Gibson assembly is required because two BbsI-digested overhang sequences are the same. Please visit https://doi.org/10.1101/2024.09.18.613662 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmU1-CasRx-non-targeting (pKW83) was a gift from S Chul Kwon (Addgene plasmid # 229051 ; http://n2t.net/addgene:229051 ; RRID:Addgene_229051)

  • For your References section:

    Cytosolic CRISPR RNAs for efficient application of RNA-targeting CRISPR-Cas systems. Cheng ECK, Lam JKC, Kwon SC. EMBO Rep. 2025 Feb 26. doi: 10.1038/s44319-025-00399-4. 10.1038/s44319-025-00399-4 PubMed 40011676
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