pHEE901-A3A
(Plasmid
#229044)
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Purpose(Empty Backbone) nCas9 mediated-base editing with NGG PAM recognition motif in Arabidopsis. A3A-nCas9-UGI includes D10A in Cas9.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229044 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepHEE901
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Vector typePlant Expression, CRISPR
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Binary vector with Hygromycin resistance in Arabidopsis and Kanamycin resistance in bacteria
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHEE901-A3A was a gift from Huawei Zhang (Addgene plasmid # 229044 ; http://n2t.net/addgene:229044 ; RRID:Addgene_229044) -
For your References section:
Creating large-scale genetic diversity in Arabidopsis via base editing-mediated deep artificial evolution. Wang X, Pan W, Sun C, Yang H, Cheng Z, Yan F, Ma G, Shang Y, Zhang R, Gao C, Liu L, Zhang H. Genome Biol. 2024 Aug 9;25(1):215. doi: 10.1186/s13059-024-03358-9. 10.1186/s13059-024-03358-9 PubMed 39123212