ePE6d
(Plasmid
#229029)
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PurposeExpresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in rice
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 229029 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonephuc
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Vector typeCRISPR
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin and Spectinomycin, 50 & 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ePE6d was a gift from Li Juan (Addgene plasmid # 229029 ; http://n2t.net/addgene:229029 ; RRID:Addgene_229029) -
For your References section:
Engineering PE6 prime editors to efficiently insert tags in rice. Xu R, Ma C, Sheng J, Zhu J, Wang D, Liu X, Wang Q, Li J, Qin R, Wei P. Plant Biotechnol J. 2024 Sep 27. doi: 10.1111/pbi.14456. 10.1111/pbi.14456 PubMed 39331467