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pRK5-HA-Ubiquitin-K27
(Plasmid #22902)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22902 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRK5-HA
  • Backbone size w/o insert (bp) 4800
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ubiquitin C
  • Alt name
    Ub
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    220
  • Mutation
    K27 only, all other lysines mutated to arginines
  • Entrez Gene
    UBC (a.k.a. HMG20)
  • Tag / Fusion Protein
    • HA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pRK5-HA-Ubiquitin-K27 was derived from pRK5-HA-Ubiquitin-K0 originally generated by the Dr. Ted Dawson laboratory (Lim KL et al. J Neuroscience. 2005. Feb 23. 25(8): 2002-9).
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5-HA-Ubiquitin-K27 was a gift from Sandra Weller (Addgene plasmid # 22902 ; http://n2t.net/addgene:22902 ; RRID:Addgene_22902)

  • For your References section:

    Virus-Induced Chaperone-Enriched (VICE) domains function as nuclear protein quality control centers during HSV-1 infection. Livingston CM, Ifrim MF, Cowan AE, Weller SK. PLoS Pathog. 2009 Oct . 5(10):e1000619. 10.1371/journal.ppat.1000619 PubMed 19816571
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