pCDNA5-LRRK2
(Plasmid #229019)

• Purpose: Expression of untagged full length human LRRK2 in mammalian cells
• Depositing Lab: Samara Reck-Peterson
• Publication: Dederer et al J Biol Chem. 2024 Jul;300(7):107469. doi: 10.1016/j.jbc.2024.107469. Epub 2024 Jun 12.

Backbone

• Vector backbone: pCDNA5
• Backbone manufacturer: Genescript
• Backbone size w/o insert (bp): 5094
• Total vector size (bp): 12678
• Vector type: Mammalian Expression
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Leucine-rich repeat kinase 2
• Alt name: LRRK2
• Species: H. sapiens (human)
• Insert Size (bp): 7584
• Mutation: none
• GenBank ID: NM_198578.4 NM_198578.4
• Entrez Gene: LRRK2 (a.k.a. AURA17, DARDARIN, PARK8, RIPK7, ROCO2)
• Promoter: CMV
• Tag / Fusion Protein:
  • no tags (untagged)

Cloning Information

• Cloning method: Gene Synthesis
• 5′ sequencing primer: Plasmidsaurus full sequencing

Resource Information

• Supplemental Documents:
  • Snapgene map of construct and full plasmid sequencing verification.
• A portion of this plasmid was derived from a plasmid made by: GeneScript. We designed and ordered it as synthesized plasmid.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Also used in : Type-II kinase inhibitors that target Parkinson’s Disease-associated LRRK2
Nicolai D. Raig, Katherine J. Surridge, Marta Sanz-Murillo, Verena Dederer, Andreas Krämer, Martin P. Schwalm, Lewis Elson, Deep Chatterjee, Sebastian Mathea, Thomas Hanke, Andres E. Leschziner, Samara L. Reck-Peterson, Stefan Knapp
bioRxiv 2024.09.17.613365; doi: https://doi.org/10.1101/2024.09.17.613365