pRK5-HA-Ubiquitin-K6
(Plasmid #22900)

• Depositing Lab: Sandra Weller
• Publication: Livingston et al PLoS Pathog. 2009 Oct . 5(10):e1000619.

Backbone

• Vector backbone: pRK5-HA
• Backbone size w/o insert (bp): 4800
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Ubiquitin C
• Alt name: Ub
• Species: H. sapiens (human)
• Insert Size (bp): 220
• Mutation: K6 only, all other lysines mutated to arginines
• Entrez Gene: UBC (a.k.a. HMG20)
• Tag / Fusion Protein:
  • HA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: SP6

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pRK5-HA-Ubiquitin-K6 was derived from pRK5-HA-Ubiquitin-K0 originally generated by the Dr. Ted Dawson laboratory (Lim KL et al. J Neuroscience. 2005. Feb 23. 25(8): 2002-9).
• Articles Citing this Plasmid:
  • 14 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry