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pME18S-3HA-mULK2(K39T)
(Plasmid #22899)

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Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 22899 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pME18s
  • Backbone size w/o insert (bp) 3400
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    UNC-51-like kinase 2
  • Alt name
    ULK2
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3500
  • Mutation
    changed Lysine 39 to Threonine
  • GenBank ID
    AB019577
  • Entrez Gene
    Ulk2 (a.k.a. RP23-278F12.5, A830085I22Rik, AU015340, Unc51.2, mKIAA0623)
  • Tag / Fusion Protein
    • 3xHA (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (destroyed during cloning)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer N/A
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pME18S-3HA-mULK2(K39T) was a gift from Masaaki Muramatsu (Addgene plasmid # 22899 ; http://n2t.net/addgene:22899 ; RRID:Addgene_22899)

  • For your References section:

    Mouse ULK2, a novel member of the UNC-51-like protein kinases: unique features of functional domains. Yan J, Kuroyanagi H, Tomemori T, Okazaki N, Asato K, Matsuda Y, Suzuki Y, Ohshima Y, Mitani S, Masuho Y, Shirasawa T, Muramatsu M. Oncogene. 1999 Oct 21. 18(43):5850-9. 10.1038/sj.onc.1202988 PubMed 10557072
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