Skip to main content
Addgene

pET21-pelB-GST-1
(Plasmid #228983)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228983 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET21a(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5443
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    GST-1
  • Species
    Lama glama
  • Insert Size (bp)
    434
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHIS (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 Term
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET21-pelB-GST-1 was a gift from Michael Rout (Addgene plasmid # 228983 ; http://n2t.net/addgene:228983 ; RRID:Addgene_228983)

  • For your References section:

    A new generation of nanobody research tools using improved mass spectrometry-based discovery methods. Fridy PC, Farrell RJ, Molloy KR, Keegan S, Wang J, Jacobs EY, Li Y, Trivedi J, Sehgal V, Fenyo D, Wu Z, Chait BT, Rout MP. J Biol Chem. 2024 Aug 2;300(9):107623. doi: 10.1016/j.jbc.2024.107623. 10.1016/j.jbc.2024.107623 PubMed 39098531
Related items: