pME18S-3HA-mULK2
(Plasmid
#22898)
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Depositing Lab
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Publication
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 22898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepME18s
- Backbone size w/o insert (bp) 3400
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameUNC-51-like kinase 2
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Alt nameULK2
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SpeciesM. musculus (mouse)
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Insert Size (bp)3500
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GenBank IDAB019577
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Entrez GeneUlk2 (a.k.a. RP23-278F12.5, A830085I22Rik, AU015340, Unc51.2, mKIAA0623)
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Tag
/ Fusion Protein
- 3xHA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME18S-3HA-mULK2 was a gift from Masaaki Muramatsu (Addgene plasmid # 22898 ; http://n2t.net/addgene:22898 ; RRID:Addgene_22898) -
For your References section:
Mouse ULK2, a novel member of the UNC-51-like protein kinases: unique features of functional domains. Yan J, Kuroyanagi H, Tomemori T, Okazaki N, Asato K, Matsuda Y, Suzuki Y, Ohshima Y, Mitani S, Masuho Y, Shirasawa T, Muramatsu M. Oncogene. 1999 Oct 21. 18(43):5850-9. 10.1038/sj.onc.1202988 PubMed 10557072
Map uploaded by the depositor.