pME18S-3HA-mULK1(K46N)
(Plasmid #22897)

• Depositing Lab: Masaaki Muramatsu
• Publication: Yan et al Biochem Biophys Res Commun. 1998 May 8. 246(1):222-7.

Backbone

• Vector backbone: pME18s
• Backbone size w/o insert (bp): 3410
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: a novel protein kinase structurally related to C. elegans UNC-51
• Alt name: ULK1
• Species: M. musculus (mouse)
• Insert Size (bp): 3614
• Mutation: changed Lysine 46 to Aspargine
• GenBank ID: AF053756
• Entrez Gene: Ulk1 (a.k.a. AU041434, Unc51.1, mKIAA0722)
• Tag / Fusion Protein:
  • 3xHA (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: N/A

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry