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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22896 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepME18s
- Backbone size w/o insert (bp) 3410
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namea novel protein kinase structurally related to C. elegans UNC-51
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Alt nameULK1
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SpeciesM. musculus (mouse)
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Insert Size (bp)3614
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GenBank IDAF053756
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Entrez GeneUlk1 (a.k.a. AU041434, Unc51.1, mKIAA0722)
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Tag
/ Fusion Protein
- 3xHA (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site NotI (not destroyed)
- 5′ sequencing primer N/A (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pME18S-3HA-mULK1 was a gift from Masaaki Muramatsu (Addgene plasmid # 22896 ; http://n2t.net/addgene:22896 ; RRID:Addgene_22896) -
For your References section:
Identification of mouse ULK1, a novel protein kinase structurally related to C. elegans UNC-51. Yan J, Kuroyanagi H, Kuroiwa A, Matsuda Y, Tokumitsu H, Tomoda T, Shirasawa T, Muramatsu M. Biochem Biophys Res Commun. 1998 May 8. 246(1):222-7. 10.1006/bbrc.1998.8546 PubMed 9600096
Map uploaded by the depositor.
