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Addgene

pWN_U6-B2M-miniGag-Cas9
(Plasmid #228958)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228958 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCAGGS
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    miniGag-Cas9

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWN_U6-B2M-miniGag-Cas9 was a gift from Jennifer Doudna (Addgene plasmid # 228958 ; http://n2t.net/addgene:228958 ; RRID:Addgene_228958)

  • For your References section:

    Mechanism-guided engineering of a minimal biological particle for genome editing. Ngo W, Peukes J, Baldwin A, Xue ZW, Hwang S, Stickels RR, Lin Z, Satpathy AT, Wells JA, Schekman R, Nogales E, Doudna JA. Proc Natl Acad Sci U S A. 2025 Jan 7;122(1):e2413519121. doi: 10.1073/pnas.2413519121. Epub 2024 Dec 30. 10.1073/pnas.2413519121 PubMed 39793042
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