pWN_U6-B2M-miniGag
(Plasmid
#228957)
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PurposeminiEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228957 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backboneAddgene Plasmid #201915
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameminiGag
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Plasmid is also known as pWN_U6-B2M-miniGag-Pol
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWN_U6-B2M-miniGag was a gift from Jennifer Doudna (Addgene plasmid # 228957 ; http://n2t.net/addgene:228957 ; RRID:Addgene_228957) -
For your References section:
Mechanism-guided engineering of a minimal biological particle for genome editing. W. Ngo, J. Peukes, A. Baldwin, Z.W. Xue, S. Hwang, R.R. Stickels, Z. Lin, A.T. Satpathy, J.A. Wells, R. Schekman, E. Nogales, J.A. Doudna. Proc. Natl. Acad. Sci. U.S.A., 122 (1), e2413519121, 2025 10.1073/pnas.2413519121