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Addgene

mCherry-STIM1
(Plasmid #228952)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228952 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    Addgene 19754
  • Backbone manufacturer
    Anjana Rao
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    STIM1
  • Species
    H. sapiens (human)
  • Entrez Gene
    STIM1 (a.k.a. D11S4896E, GOK, IMD10, STRMK, TAM, TAM1)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Retroviral construct for mCherry-STIM1 expression was cloned by replacing the DNA sequence of YFP in the retroviral YFP-STIM1 plasmid (Addgene plasmid 19754) with the DNA sequence of mCherry

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCherry-STIM1 was a gift from David Pellman (Addgene plasmid # 228952 ; http://n2t.net/addgene:228952 ; RRID:Addgene_228952)

  • For your References section:

    A tubule-sheet continuum model for the mechanism of nuclear envelope assembly. Zhao G, Liu S, Arun S, Renda F, Khodjakov A, Pellman D. Dev Cell. 2023 May 22;58(10):847-865.e10. doi: 10.1016/j.devcel.2023.04.003. Epub 2023 Apr 24. 10.1016/j.devcel.2023.04.003 PubMed 37098350
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