pcDNA4-Chk1-Flag
(Plasmid #22894)

• Depositing Lab: Aziz Sancar
• Publication: Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Backbone

• Vector backbone: pcDNA4a
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 5100
• Vector type: Mammalian Expression
• Selectable markers: Zeocin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Chk1
• Alt name: CHEK1
• Species: H. sapiens (human)
• Insert Size (bp): 1430
• GenBank ID: AK300082.1
• Entrez Gene: CHEK1 (a.k.a. CHK1, OZEMA21)
• Tag / Fusion Protein:
  • Flag (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (unknown if destroyed)
• 3′ cloning site: PmeI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Stephen Elledge; pBSChk1
• Articles Citing this Plasmid:
  • 7 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry