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pMRX-IP-EGFP-PA-BAX(lyso)
(Plasmid #228939)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228939 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pMRX-IP
  • Total vector size (bp) 8333
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    DH5alpha will also be fine
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    LOV2 domain of phototropin1
  • Species
    Avena sativa
  • Insert Size (bp)
    441
  • Mutation
    N538E
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Gene/Insert 2

  • Gene/Insert name
    BAX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    507
  • Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)
  • Entrez Gene
    BAX (a.k.a. BCL2L4)

Gene/Insert 3

  • Gene/Insert name
    TMEM106B
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    558
  • Mutation
    N-terminus is deleted (TMEM106B 90-274)
  • Entrez Gene
    TMEM106B (a.k.a. HLD16)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.08.13.607738 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMRX-IP-EGFP-PA-BAX(lyso) was a gift from Noboru Mizushima (Addgene plasmid # 228939 ; http://n2t.net/addgene:228939 ; RRID:Addgene_228939)

  • For your References section:

    Optogenetic tools for inducing organelle membrane rupture. Nagashima Y, Eguchi T, Koyama-Honda I, Mizushima N. J Biol Chem. 2025 Mar 18;301(4):108421. doi: 10.1016/j.jbc.2025.108421. 10.1016/j.jbc.2025.108421 PubMed 40113041
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