pRDA_174_neoR
(Plasmid
#228935)
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Purpose(Empty Backbone) Lentiviral expression of EnAsCas12a
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228935 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepRDA_174
- Backbone size (bp) 12177
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pRDA_174_neoR was a gift from Francisca Vazquez (Addgene plasmid # 228935 ; http://n2t.net/addgene:228935 ; RRID:Addgene_228935) -
For your References section:
SKI complex loss renders 9p21.3-deleted or MSI-H cancers dependent on PELO. Borck PC, Boyle I, Jankovic K, Bick N, Foster K, Lau AC, Parker-Burns LI, Lubicki DA, Li T, Borah AA, Lofaso NJ, Das Sharma S, Chan T, Kishen RV, Adeagbo A, Raghavan S, Aquilanti E, Prensner JR, Krill-Burger JM, Golub TR, Campbell CD, Dempster JM, Chan EM, Vazquez F. Nature. 2025 Feb 5. doi: 10.1038/s41586-024-08509-3. 10.1038/s41586-024-08509-3 PubMed 39910293