pcDNA3-Flag-RPA2
(Plasmid #22893)

• Depositing Lab: Aziz Sancar
• Publication: Unsal-Kacmaz et al Mol Cell Biol. 2007 Apr . 27(8):3131-42.

Backbone

• Vector backbone: pcDNA3
• Backbone size w/o insert (bp): 5400
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: RPA2
• Alt name: RPA34
• Species: H. sapiens (human)
• Insert Size (bp): 810
• Entrez Gene: RPA2 (a.k.a. REPA2, RP-A p32, RP-A p34, RPA32)
• Tag / Fusion Protein:
  • Flag (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: XbaI (unknown if destroyed)
• 5′ sequencing primer: T7

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

There is one point mutation in the insert, but the protein expressed with this construct forms complexes with the other subunits of the RPA heterotrimer, binds other known interacting proteins (XPA, Tipin), and shows an enrichment in the chromatin fraction of cells treated with replication stress-inducing agents. So by these criteria the construct appears to be WT.