pFastBac-His-Flag-Timeless
(Plasmid
#22892)
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Depositing Lab
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Sequence Information
Full plasmid sequence is not available for this item.
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 22892 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepFastBacHTb
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 4856
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Vector typeInsect Expression
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Selectable markersGentamicin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameTimeless
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SpeciesH. sapiens (human)
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Insert Size (bp)3540
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Entrez GeneTIMELESS (a.k.a. FASPS4, TIM, TIM1, hTIM)
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Tags
/ Fusion Proteins
- His (N terminal on backbone)
- Flag (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site XbaI (unknown if destroyed)
- 3′ cloning site KpnI (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFastBac-His-Flag-Timeless was a gift from Aziz Sancar (Addgene plasmid # 22892 ; http://n2t.net/addgene:22892 ; RRID:Addgene_22892) -
For your References section:
The human Tim/Tipin complex coordinates an Intra-S checkpoint response to UV that slows replication fork displacement. Unsal-Kacmaz K, Chastain PD, Qu PP, Minoo P, Cordeiro-Stone M, Sancar A, Kaufmann WK. Mol Cell Biol. 2007 Apr . 27(8):3131-42. 10.1128/MCB.02190-06 PubMed 17296725