pJCX3-ACTG2_R257C
(Plasmid
#228911)
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PurposeExpresses mutant R257C smooth muscle actin gamma-2 in human cells
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 228911 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepJC
- Backbone size w/o insert (bp) 4700
- Total vector size (bp) 5800
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Vector typeMammalian Expression, Bacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameActin gamma 2, smooth muscle
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Alt nameACTG2
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SpeciesH. sapiens (human); Human
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Insert Size (bp)1120
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MutationR257C
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GenBank IDNM_001615.4 NM_001615.4
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Entrez GeneACTG2 (a.k.a. ACT, ACTA3, ACTE, ACTL3, ACTSG, MMIHS5, VSCM, VSCM1)
- Promoter CMV
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Tag
/ Fusion Protein
- HIS-FLAG-TEV (N terminal on insert)
Cloning Information
- Cloning method Other
- 5′ sequencing primer TAGGCGTGTACGGTGGG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pJCX3-ACTG2_R257C was a gift from Roberto Dominguez (Addgene plasmid # 228911 ; http://n2t.net/addgene:228911 ; RRID:Addgene_228911)