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Addgene

pJCX3-ACTG2_WT
(Plasmid #228910)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 228910 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pJC
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 5800
  • Vector type
    Mammalian Expression, Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Actin gamma 2, smooth muscle
  • Alt name
    ACTG2
  • Species
    H. sapiens (human); Human
  • Insert Size (bp)
    1120
  • Mutation
    WT
  • GenBank ID
    NM_001615.4 NM_001615.4
  • Entrez Gene
    ACTG2 (a.k.a. ACT, ACTA3, ACTE, ACTL3, ACTSG, MMIHS5, VSCM, VSCM1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HIS-FLAG-TEV (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI (not destroyed)
  • 3′ cloning site Bcl1 (destroyed during cloning)
  • 5′ sequencing primer TAGGCGTGTACGGTGGG
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJCX3-ACTG2_WT was a gift from Roberto Dominguez (Addgene plasmid # 228910 ; http://n2t.net/addgene:228910 ; RRID:Addgene_228910)
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